Business competition in the global era requires companies to be able to achieve effectiveness and efficiency in every process. However, in processing HPBE products CAI still indicated off quality by findings in May 2021, where as much as 55.6% were off spec for pH levels, 22.2% off spec for acidity levels, and 22.2% off spec for H2O content. This indicates a decrease in the effectiveness of HPBE production. The program is taken by CAI company in order to overcome various wastes that occur at the process stages by implementing a continuous improvement program through a 5S culture in the work area. This research was conducted to determine the effect of 5S culture on production effectiveness at PT. CAI used a sample of 100 respondents. Methods of data collection through observation and questionnaires. The results showed that partially Seiri, Seiton, Shitsuke, and Seiketsu had a significant effect on the effectiveness of BE production. While Seiso partially has no significant effect on production effectiveness. However, Seiri, Seiton, Seiso, Shitsuke, and Seiketsu together have a significant effect on production effectiveness. The culture of 5S implementation can increase the effectiveness of HPBE production, therefore programs to improve attitudes towards the implementation of 5S culture in the work area must be given more attention by every level of management.