Staphylococcus aureus (S. aureus) resistant to Methicillin (MRSA) occupies a significant place in modern microbiology and infection control procedures. The frequency of MRSA infections continues to grow in hospital and community associated settings. The increase in the incidence of infections due to S. aureus is a consequence of the pathogen’s ability to adapt to a changing environment. Methods: Analyzes were performed on 48 samples of Staphylococcus strains from farm animals, exposed and non-exposed personnel obtained from the Université des Frères Mentouri (Constantine) and Institute des Sciences Vétérinaires El Khroub (Algeria), collected during 2016. Each strain was tested against 30 antibiotics to detect antibiotic resistance. Results: Among 48 tested samples 41 (85,4%) were identified as S. aureus. Our results revealed that 14,6% of the S. aureus strains were resistant to Cefoxitin, which would presumably indicate MRSA strains. We also confirmed resistance to Methicillin, Penicillin, Oxacillin resistance and the majority of isolated strains were multi-resistant. Conclusion: These results confirmed that antibiotics resistance is present not only in nosocomial bacteria, but also in livestock and community environments microorganisms. The majority of isolated S. aureus strains were multi-resistant to more than three classes of antibiotics. MRSA strains were detected in 14,6% of our samples and was common among samples from farmers and non-exposed students.