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The Use of Domestic Waste as Culture Media of Bacillus Thuringensis for Minimizing Pre-Adult Mosquitoes Population

Pratiwi Hermiyanti, Fitri Rokhmalia and Ngadino
Keywords: Domestic waste, Killing power of Bacillus thuringiensis spores, Mosquito Larvae.

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Hermiyanti, P., Rokhmalia, F. and Ngadino, 2021. The Use of Domestic Waste as Culture Media of Bacillus Thuringensis for Minimizing Pre-Adult Mosquitoes Population. United International Journal for Research & Technology (UIJRT), 2(4), pp.11-15.

Abstract

The use of bacteria as bio insecticide is safe, effective, and selective in eradicating mosquito larvae, especially Aegypti mosquito larvae. Domestic waste (black water) contains high protein that can be nutrient for development of Bacillus thuringensis as biolarvacide. Nevertheless, the purpose of this study was in order to analyze the effectiveness of using domestic waste as culture media of Bacillus thuringensis for minimizing Aedes mosquito larvae population.

Design of this research was experimental with posttest only control group design. The sample size of mosquito larvae that was used in this research was 160 larvae and each 20 larvae was in each dose of treatment and control. Suspension dose of liquid waste media of Bacillus thuringensis culture that was used in this treatment was 1 ml; 2 ml; 3 ml; 5 ml; 7 ml; 10 ml; 30 ml; and 50ml. Data analysis was by analyzing table and it was presented in narrative.

The content of domestic waste that supported as nutrient for Bacillus thuringensis was carbohydrate (0.88%), protein (1.62%), and the state of waste pH (pH 7). Activity of Bacillus thuringensis was less optimal to develop in domestic liquid waste media. This was indicated by the absence of white sediment at the bottom of Erlenmeyer flask which showed the development of bacteria. Liquid waste media that was cultured by Bacillus thuringensis did not show any significant effectiveness to become biolarvacides because no doses which were applied could eradicate mosquito larvae.

All in all, conclusion of this research was Bacillus thuringensis had not been optimally developed in domestic liquid waste media, hence, its pathogenicity could not be tested on mosquito larvae.

References

  1. Gazali, A. Jaelani. Patogenicity of Bacillus thuringensis which Isolated from Tidal Ecosystem against Diamond Backmoth Larvae, Plutella xylostella Linn. Asian Journal of Applied Sciences, 2015, 3(3), pp. 513-518.
  2. Gazali, A. Jaelani. Bacillus thuringensis Berliner Cells Population Growth in Some Naturally Media and the Patogenecity Against Plutella xylostella Caterpilars. Asian Journal of Applied Science, 2017.
  3. Akhmad Gazali, Ilhamiyah. A. Bacillus thuringensis: Biologi, Isolasi, Perbanyakan dan Cara Aplikasinya. Banjarmasin: Pustaka Banua, 2017.
  4. Ariyani, Nurita Ika Milasari dan Sukma Budi. Pengolahan Limbah Cair Kadar COD dan Fenol Tinggi dengan Proses Anaerob dan Pengaruh Mikronutrient Cu: Kasus Limbah Industri Jamu Tradisional. Semarang: Universitas Diponegoro, 2010.
  5. Blondine, Lulus Susanti. Efikasi Bacillus thuringensis H-14 yang Dibiakan dalam Media Kelapa pada Penyimpanan Suhu Kamar dan Refrigerator (Suhu 40 C) terhadap Vektor DBD dan Malaria. Jurnal Vektora, 2009, 1(2), pp. 109-122.
  6. Dahruji, Pipit Festi Wilianarti, Totok Hendarto. Studi Pengolahan LImbah Usaha Mandiri Rumah Tangga dan Dampak bagi Kesehatan di Wilayah Kenjeran. Jurnal Pengabdian kepada Masyarakat, 2017, 1(1), pp. 38.
  7. Hermiyanti, Pratiwi; Rokhmalia, Fitri; Darjati. Tripikon Modification as an Infiltration Ditch of Domestic Waste at Narrow Field. International Journal of Current Research, 2019, 11(7) pp.5135-5138.
  8. Mardihusodo, SJ. Sensitivitas Larva Nyamuk Aedes aegypti L. Terhadap Bacillus thuringiensis H-14 dan Bacillus sphaericus, 2012
  9. H. Doraja, Maya Shovitri, dan N.D. Kuswytasari. Biodegradasi Limbah Domestik dengan Menggunakan Inokulum Alami dari Tangki Septik. Jurnal Sains dan Seni ITS, 2012, 1(1), pp. 44-47.
  10. Puji Lestari, Rr Ni Ratih Hardisari dan Sujono. Perbedaan Angka Kuman Udara Sebelum dan Sesudah Penyinaran Lampu Ultraviolet 90 watt di Laboratorium Bakteriologi Jurusan Analisis Kesehatan Poltekkes Kemenkes Yogyakarta. Yogyakarta: Poltekkes Kemenkes Yogyakarta, 2019.
  11. Pujiastuti, Yulia; Triyansyah; Hamidson, Harman; Effendy; Suparman. Produksi Spora Bacillus Thuringiensisada Media Limbah dengan Penambahan Tepung Cangkang Keong Mas dan Toksisitasnya terhadap Spodoptera Litura Fabr. (Lepidoptera: Noctuidae). Journal of Suboptimal Land, 2017, 6(2) pp.150-157.
  12. Purnama, Sang Gede; Pandy, Deny Silvina; Sudiana, I Gede. Pemanfaatan Limbah Cair Industri Pengolahan Tahu untuk Memproduksi Spora Bacillus thuringensis Serovar israelensis dan Aplikasinya sebagai Biokontrol Larva Nyamuk. Arc. Com. Health, 2012, 1(1) pp. 1-9.
  13. Rini Purnawati, Titi C. Sunarti, Khaswar Syamsu, Mulyorini Rahayuningsih. Produksi Bioinsektisida oelh Bacillus thuringensis Menggunakan Kultivasi Media Padat. Jurnal Teknologi Industri Pertanian, 2015, 25(3), pp. 205-2014.
  14. Sari, Enggrit Ariana; Koerniasari; Jauhari, Soekiran Al. Perbandingan Efektivitas Penurunan Kadar BOD dan COD pada Limbah Cair Industri Tahu menggunakan Tanaman Kayu Apu (Pistia stratiotes) dan Tanaman Eceng Gondok (Eichornia crassipes Solms). GEMA Lingkungan Kesehatan, 2016, 14(2) pp. 109-113.
  15. Dasar-dasar Pengelolaan Limbah. Jakarta: UI Press, 2008.
  16. WHO, World Heath Organization. Data sheet on the biological control agent, Bacillus thuringiensis serotype H-14.WHO/VBC/79.750. 1979.
  17. Yempita Efendi, Y. Optimasi Potensi Bakteri Bacillus subtilis sebagai Sumber Enzim Protease. Jurnal Akuatika Indonesia, 2017, 2(1), pp. 87-94.

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