HIV is a virus that attacks the body’s immune system. If HIV is not treated, it can lead to AIDS. There is currently no effective cure. Purpose: The purpose of the study is to assess the attitude regarding HIV/AIDS among Secondary School Students in Bangladesh. The study was a descriptive cross-sectional design. Using convenient sampling technique 120 adolescent were recruited from two Govt. High Schools at Dhaka city. A self-structured questionnaire (1) Demographic Questionnaires, (2) Secondary School Students Attitude regarding HIV/AIDS Questionnaires were used to collect the data. Data analysis using SPSS version 22. The Secondary School Students age was ≤ 15 (74.16%) and ≥ 16 (25.84%). Secondary School Students possess positive attitude in this study. Highest five strongly agree Statement was HIV/AIDS is a threatening illness among adolescent age group 79.2%, It is necessary to learn about HIV/AIDS in school 71.7%, I can protect myself from HIV/AIDS obeying religious rules 68.3%, I will empathetic to person HIV/AIDS 65.8% and I can protect myself from HIV/AIDS by maintaining moral commitment 62.5%. This study findings, students had a positive level of attitudes regarding AIDS. Based on the study result school can provide HIV/AIDS topic in their curriculum to improved attitude. Students had positive level of attitude on HIV/AIDS infection as well as prevention methods were showed in this study. That the Ministry of Education implements awareness creation on the Infection, transmission and prevention of HIV/AIDS in secondary schools.